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unprocessed human bone marrow aspirate  (Lonza)


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    Structured Review

    Lonza unprocessed human bone marrow aspirate
    (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from <t>BMA</t> and injected into (C) perfusion <t>bioreactors</t> containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.
    Unprocessed Human Bone Marrow Aspirate, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/unprocessed+human+bone+marrow+aspirate/unprocessed+human+bone+marrow+aspirate/pmc06478553-68-5-15
    Average 90 stars, based on 1 article reviews
    unprocessed human bone marrow aspirate - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts"

    Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts

    Journal: Biomaterials science

    doi: 10.1039/c8bm01478g

    (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from BMA and injected into (C) perfusion bioreactors containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.
    Figure Legend Snippet: (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from BMA and injected into (C) perfusion bioreactors containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.

    Techniques Used: Control, Injection

    (A) Immunophenotype of unprocessed BMA and cells entrapped in scaffolds. (B – E) Percentage of entrapped cells that are (B) hematopoietic cells (CD45+), (C) hematopoietic progenitor cells (CD45+CD34+), (D) endothelial cells (CD31+CD45−), and (E) mesenchymal stem cells (CD73+CD90+CD45−) shown as a fold change to the percentage found in fresh BMA (n=4 replicates from 1 biological donor).
    Figure Legend Snippet: (A) Immunophenotype of unprocessed BMA and cells entrapped in scaffolds. (B – E) Percentage of entrapped cells that are (B) hematopoietic cells (CD45+), (C) hematopoietic progenitor cells (CD45+CD34+), (D) endothelial cells (CD31+CD45−), and (E) mesenchymal stem cells (CD73+CD90+CD45−) shown as a fold change to the percentage found in fresh BMA (n=4 replicates from 1 biological donor).

    Techniques Used:

    Related Articles

    Control:

    Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts
    Article Snippet: were sterilized by 70% ethanol under gentle vacuum followed by two rinses in sterile PBS. .. Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years). .. BMA was diluted 1:5 in PBS and pelleted by centrifugation at 1,000x g . Cells were incubated in a r

    Injection:

    Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts
    Article Snippet: were sterilized by 70% ethanol under gentle vacuum followed by two rinses in sterile PBS. .. Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years). .. BMA was diluted 1:5 in PBS and pelleted by centrifugation at 1,000x g . Cells were incubated in a r



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    Image Search Results


    (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from BMA and injected into (C) perfusion bioreactors containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.

    Journal: Biomaterials science

    Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts

    doi: 10.1039/c8bm01478g

    Figure Lengend Snippet: (A) Control media or media containing equal quantities of collagen or cell-secreted extracellular matrix (ECM) were mixed with (B) mononucleated cells from BMA and injected into (C) perfusion bioreactors containing HA-PLG scaffolds press-fit into the perfusion chamber. After 20 hours of oscillating perfusion, scaffolds were retrieved and (D) implanted into subcutaneous pockets in the back of nude rats.

    Article Snippet: Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years).

    Techniques: Control, Injection

    (A) Immunophenotype of unprocessed BMA and cells entrapped in scaffolds. (B – E) Percentage of entrapped cells that are (B) hematopoietic cells (CD45+), (C) hematopoietic progenitor cells (CD45+CD34+), (D) endothelial cells (CD31+CD45−), and (E) mesenchymal stem cells (CD73+CD90+CD45−) shown as a fold change to the percentage found in fresh BMA (n=4 replicates from 1 biological donor).

    Journal: Biomaterials science

    Article Title: Cell-secreted extracellular matrix influences cellular composition sequestered from unprocessed bone marrow aspirate for osteogenic grafts

    doi: 10.1039/c8bm01478g

    Figure Lengend Snippet: (A) Immunophenotype of unprocessed BMA and cells entrapped in scaffolds. (B – E) Percentage of entrapped cells that are (B) hematopoietic cells (CD45+), (C) hematopoietic progenitor cells (CD45+CD34+), (D) endothelial cells (CD31+CD45−), and (E) mesenchymal stem cells (CD73+CD90+CD45−) shown as a fold change to the percentage found in fresh BMA (n=4 replicates from 1 biological donor).

    Article Snippet: Seeding bone marrow aspirate in perfusion bioreactors Unprocessed human bone marrow aspirate (BMA) was from Lonza (10 mL of aspirate from n = 2 male and n = 2 female donors; 23–32 years old, mean age 26 ± 4 years).

    Techniques: